Immunology Services

SRI provides clients with high-quality immunology services. Our laboratories are equipped with state-of-the-art resources for analytical and functional studies. Class III containment facilities for bacterial culture and for the study of animal diseases, and modern equipment including fluorescent activated (FACS) and magnetic (MACS) cell sorting systems, are available. The flow cytometry facility is equipped with a BectonDickinson FACSCalibur and a Coulter ELITE cytometer. The group has extensive expertise in each assay offered and the staff is readily available to assist with the design and execution of complex immunological protocols.

Flow Cytometry Services

• Ca²⁺ flux analysis
• Cell cycle and kinetic analysis
• Multivariate (three- and four-color) surface and cytoplasmic immunophenotyping
• Measurement of DNA and RNA content
• Specific S-phase labeling of activated cells
• Detection of green fluorescent protein (GFP) expression
• Apoptosis detection
• Cytometric bead arrays
• MHC-I/peptide screening
• Receptor/peptide binding

In Vitro Bioassays

• Natural killer (NK) cell Assays
• Cytotoxic T-lymphocyte assays
• Antigen-specific functional lymphoproliferative assays
• Mixed lymphocyte cultures
• Cytokine assays (ELISA and intracellular flow cytometric analysis)
• Single cell cytokine assays (ELISPOT)
• DNA synthesis
• Differentiation
• Apoptosis
• Immunoblotting technologies
• Antigen uptake, processing and presentation
• Viral assays

Cellular Immunological Assays

• Large-scale human lymphocyte activation
• Long-term cultures of T-cells (1-2 months)
• Enriched monocyte preparation from whole blood cells
• Preparation of monocyte-derived dendritic cells
• Enriched Pan T-cell preparation from whole blood cells
• Enriched B-cell preparation from whole blood cells
• Generation of antigen-specific T-cell clones

In Vivo Bioassays

• Murine collagen-induced arthritis
• Cytokine and cytokine receptor measurement

Vaccine Efficacy

• CD4⁺ and CD8⁺ T-cell-mediated responses
  • Proliferation
  • Cytotoxicity
• Antibody response and specificity verification
  • Qualitative or quantitive ELISA
  • Chemifluorescent immunoblotting
  • Potency assay

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       Last Updated Jul 17, 2008