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In Vitro Absorption and Metabolism Models

In vitro models to predict drug absorption and metabolism are valuable techniques for both screening new drug candidates, and later evaluations of potential drug-drug interactions. As a recognized leader in the field, SRI is an obvious choice to conduct these evaluations for clients. We offer the following services, among others:

• Predict gastrointestinal absorption, using Caco2 cells.
  • Rapid screening in multi well plates, coupled to LC/MS detection detection, to quickly identify those compounds that can cross the GI tract into the blood stream.
  • Mechanism studies, evaluating flux rate and permeability constants from the apical to basolateral, and basolateral to apical directions.
  
  
  
• Evaluate drug metabolism and drug interactions, using human and animal tissue models, as recommended by FDA. We have a bank of over 100 human liver specimens for these studies and ready access to fresh human hepatocytes.
  • Determine the metabolite profile of drug candidates using hepatocytes, liver and small intestine microsomes and S9, microsomes containing a single enzyme. These samples are rapidly analyzed by using LC-MS/MS and NMR.
  • Identify Phase I or Phase II enzymes responsible for metabolite formation.
  • Predict drug interactions due to inhibition or induction of drug metabolizing enzymes.
  • Identify pharmacogenetic effects on human drug metabolism
  
  
  
• Study the transport properties of your drug, using bile canalicular membrane vesicles from animals and humans.

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       Last Updated Jan 25, 2010