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Kostas Kalogerakis

...atmospheres. He has also worked with Gregory Faris, Ph.D. on nonlinear spectroscopy techniques to study stimulated scattering in supercritical fluids, and on biomedical and microfluidics projects. During his graduate studies...

Article March 1, 2014

Microdroplet Temperature Calibration Via Thermal Dissociation of Quenched DNA Oligomers

...we present a three-term sigmoid model that provides a very good match to the asymmetric fluorescence melting curve with premelting. Using mixtures of three oligomers of different lengths, we fit...

Article January 1, 2011

Light Driven Formation of Adhesive Emulsion Drops to Form Lipid Bilayers

We demonstrate optical manipulation of nanoliter aqueous droplets containing surfactant or lipid molecules and immersed in an organic liquid using near infrared light. The resulting emulsion droplets are manipulated using...

Article April 1, 2010

Light-Driven Formation and Rupture of Droplet Bilayers

We demonstrate optical manipulation of nanoliter aqueous droplets containing surfactant or lipid molecules and immersed in an organic liquid using near infrared light. The resulting emulsion droplets are manipulated using...

Conference Paper June 1, 2009

Near-Infrared Fluorescent Labeling of Tissue Transglutaminase Substrates for Wound Healing Monitoring

A novel imaging strategy is developed to optically monitor the wound healing process by crosslinking near-infrared fluorescent-labeled tissue transglutaminase substrates into the wound....

Article April 1, 2009

Development of a Transillumination Infrared Modality for Differential Vasoactive Optical Imaging

We present the development and implementation of a new near infrared transillumination imaging modality for tissue imaging. Exogenous inhaled hyperoxic and hypercarbic gases are used as “vasoactive contrast agents” via...

Article January 1, 2009

Frequency Domain Measurements on Turbid Media with Strong Absorption Using the Pn Approximation

...a modified scattering coefficient of 4.3 cm−1 are studied, which corresponds to a reduced scattering albedo of 0.65. Low phase noise and good phase stability are required for these low...

Conference Paper January 1, 2009

Laser Heated Nanodroplet PCR on a Petri Dish

We report the development of a real-time PCR system driven solely by laser heating with nanoliter droplets on a polystyrene Petri dish....

Article January 1, 2009

Nanodroplet Real-Time PCR System with Laser Assisted Heating

...No microheaters or microfluidic circuitry were deposited on the substrate, and PCR was performed in one droplet without affecting neighboring droplets. The assay performance was quantitative and its amplification efficiency...

Article January 1, 2009

Petri Dish PCR: Laser-Heated Reactions in Nanoliter Droplet Arrays

...shape. The uniform droplet array was used to optimize the laser power required for the two heating steps of PCR, annealing/extension and melting, while the ambient conditions were at room...

Conference Paper May 1, 2008

Near-Infrared Fluorescent Labeling of Tissue Transglutaminase Substrates for Imaging Tumor Boundaries

A novel strategy is developed to optically image tumor boundaries by cross-linking near-infrared fluorescent-labeled tissue transglutaminase substrates into tumor boundary tissue....

Article January 1, 2008

Inspiratory Contrast for in Vivo Optical Imaging

We demonstrate the use of inspired oxygen and carbon dioxide as a possible route to increase contrast in optical imaging of cancerous tissue. Differential imaging in human xenograft rodent models...

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