Proteomics | SRI International

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Proteomics—the study of the proteins that make up living organisms and infectious agents—augments our ability to treat disease. An emerging and important area of systems biology, proteomics involves system-wide quantitation of the proteins present in any cell or tissue sample that exhibits a particular phenotype of interest. Large proteome analysis has exciting applications in biomarker discovery, host-pathogen interactions, studies of disease mechanism, and monitoring the effects of drug treatment.

SRI Biosciences engages in independent, collaborative, or sponsored proteomics research in all of these areas. Current interests include biomarker discovery and platform technology development for quantitative whole proteome analysis of cells and tissues.

Our proteomic studies fill in gaps in scientific understanding of the biological systems critical to defense from infection. This new understanding is facilitated by in vitro and in vivo laboratory experiments and in silico computational biology. We apply the knowledge gained to discovering and developing new treatments, cures, and countermeasures to bioterror threats.

Proteomics is also at the core of SRI Biosciences' work to detect, prevent, and treat neglected tropical diseases such as dengue fever, Leishmaniasis, and West Nile virus. Our researchers conduct proteomics studies of host-vector-pathogen interactions, focusing on insect-borne viruses, respiratory and diarrheal pathogens, and parasitic protozoa. They investigate how pathogens evade or disable the defenses of their hosts; then, to create new diagnostics, therapeutics, and vaccines, they isolate and identify compounds from insects that have antiviral or antiparasitic effects.

Comprehensive Capabilities

  • Biomarker discovery in biological fluids, such as serum, plasma, urine, and lung fluid
  • Quantitative biomarker discovery using tandem mass tag labeling with up to 6-plex analysis (optional label-free quantitation also available)
  • Custom designed multiple search engine analyses to maximize the number of identified proteins and post-translational modifications
  • Biomarker validation via triple quadrupole-based multiple reaction monitoring
  • Identification and analysis of glycoprotein biomarkers
  • Subtractive proteomics analysis of protein complexes and complex affinity extracts
  • Mass spectrometry instrumentation, including automated online Orbitrap XL-ETD capillary 2D-LC-MS/MS