Riley, M., and Abe, T., and Arnaud, M.B., and Berlyn, M.K.B., and Blattner, F.R., and Chaudhuri, R.R., and Glasner, J.D., and Horiuchi, T., and Keseler, I.M., and Kosuge, T., and Mori, H., and Perna, N.T., and Plunkett, G.3rd, and Rudd, K.E., and Serres, M.H., and Thomas, G.H., and Thomson, N.R., and Wishart, D., and Wanner, B.L. Escherichia coli K-12: a cooperatively developed annotation snapshot 2005. Nucleic Acids Research, vol. 34, no. 1, pp. 1-9, 2006.
Abstract
iV-Acetylneuraminate lyase [Ar-acetylneuraminic acid aldolase EC 4.1.3.3] from Escherichia coli was purified by protamine sulfate treatment, fractionation with ammonium sulfate, column chromatography on DEAE-Sephacel, gel filtration on Ultrogel AcA 44, and preparative polyacrylamide gel electrophoresis. The purified enzyme preparation was homogeneous on analytical polyacrylamide gel electrophoresis, and was free from contaminating enzymes including NADH oxidase and NADH dehydrogenase.
