Beaulieu, E. D., Olson, L. L., & Tanga, M. J. (2011). A platform for the detection of trypanosomes via selective small molecule recognition. ACS Medicinal Chemistry Letters, 2(7), 555-558.
Abstract
Trypanothione (TSH2), a metabolite unique to trypanosomal parasites, was evaluated as a potential biomarker for trypanosomal infection using fluorescence as the means of detection. Fluoroescein arsenical helix binder (FLASH) was prepared and used to detect TSH2. Since it has low background fluorescence and forms a highly emissive complex with TSH2, it can be used to detect low micromolar concentrations of TSH2 in serum. The large dynamic range of FLASH and its selectivity for detection of the dithiol metabolite indicate that arsenical probes may offer a promising new platform for the diagnosis of trypanosomal infection.
Keywords: Trypanosomes, trypanothione, Chagas’ disease, human African trypanosomiasis, African sleeping sickness, leishmaniasis, diagnostic, fluorescein arsenical helix binder (FLASH)