Caspi R, Helinski DR, Pacek M, Konieczny I. Interactions of DnaA proteins from distantly related bacteria with the replication origin of the broad host range plasmid RK2. J. Biol. Chem., vol. 275, no. 24, pp. 18454-18461, Jun 2000.
Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin (oriV). In contrast to a chromosomal replication origin, which functionally interacts only with the native DnaA protein of the organism, the ability of RK2 to replicate in a wide range of Gram-negative bacterial hosts requires the interaction oforiV with many different DnaA proteins. In this study we compared the interactions of oriV with five different DnaA proteins.