Development and validation of a method to extract and quantitate paromomycin and gentamicin from an Aquaphillic® cream formulation

Abstract

Butanol and dilute sulfuric acid were used to extract paromomycin and gentamicin from Aquaphilic^®-based formulated creams. The extraction procedure was validated over different antibiotic concentration ranges for linearity, precision, accuracy, limited specificity, sensitivity and solution stability.