Citation
Hundt, W., Steinbach, S., Mayer, D., & Bednarski, M. D. (2009). Modulation of luciferase activity using high intensity focused ultrasound in combination with bioluminescence imaging, magnetic resonance imaging and histological analysis in muscle tissue. Ultrasonics, 49(6-7), 549-557.
Abstract
This study investigates the effect of high intensity focused ultrasound (HIFU) to muscle tissue transfected with a luciferase reporter gene under the control of a CMV-promoter. HIFU was applied to the transfected muscle tissue using a dual HIFU system. In a first group four different intensities (802 W/cm2, 1401 W/cm2, 2117 W/cm2, 3067 W/cm2) of continuous HIFU were applied 20 s every other week for four times. In a second group two different intensities (802 W/cm2, 1401 W/cm2) were applied 20 s every fourth day for 20 times. The luciferase activity was determined by bioluminescence imaging. The effect of HIFU to the muscle tissue was assessed by T1-weighted ± Gd-DTPA, T2-weighted and a diffusion-weighted STEAM sequence obtained on a 1.5-T GE-MRI scanner. Histology of the treated tissue was done at the end. In the first group the photon emission was at 3067.6 W/cm2 1.28 × 107 ± 3.1 × 106 photon/s (5.5 ± 1.2-fold), of 2157.9 W/cm2 8.1 ± 2.7 × 106 photon/s (3.2 ± 1.1-fold), of 1401.9 W/cm2 9.3 ± 1.3 × 106 photon/s (4.9 ± 0.4-fold) and of 802.0 W/cm2 8.6x ± 1.2 × 106 photon/s (4.5 ± 0.6-fold) compared to baseline. In the second group the photon emission was at 1401.9 W/cm2 and 802.0 W/cm2 14.1 ± 3.6 × 106 photon/s (6.1 ± 1.5-fold), respectively, 5.1 ± 4.7 × 106 photon/s (6.5 ± 2.0-fold). HIFU can enhance the luciferase activity controlled by a CMV-promoter.
